This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
In the months before, space agency officials were in frequent contact with the State Department, which disseminated the latest predicted trajectories to embassies across the world. In these situations, oops doesn’t cut it: When one of the Salyuts, a Soviet space station model, was deorbited a few decades ago, flaming bits were littered across Argentina, scaring people and requiring the deployment of at least a few firefighters, according to local newspaper reports.
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